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Immune responses elicited by apoB-100-derived peptides in mice

Author:
  • Chrysoulla Pierides
  • Alexandra Bermudez-Fajardo
  • Gunilla Nordin Fredrikson
  • Jan Nilsson
  • Ernesto Oviedo-Orta
Publishing year: 2013
Language: English
Pages: 96-108
Publication/Series: Immunologic Research
Volume: 56
Issue: 1
Document type: Journal article
Publisher: Humana Press

Abstract english

Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised animals. We also measured the effect of immunisation on the number of spleen-derived CD4(+) and CD8(+) regulatory T cells (Tregs) in these animals. Peptide and peptide-loaded DC immunisation significantly increased the levels of peptide-specific immunoglobulins and the number of Tregs in apoE(-/-) mice. This was accompanied by a significant increase in the secretion of IL-10 with no effect on IFN-gamma levels. The results also show that the peptides can modulate the homing properties of DCs. Altogether, this study provides novel evidence for the immune mechanisms excerpted by apoB-100-derived peptides and their effect on Tregs and DCs relevant to their use in vaccine preparations.

Keywords

  • Cardiac and Cardiovascular Systems
  • Peptides
  • Dendritic cells
  • Regulatory T cells
  • Lymphocyte
  • Immune
  • response

Other

Published
  • Cardiovascular Research - Immunity and Atherosclerosis
  • ISSN: 0257-277X
E-mail: jan [dot] nilsson [at] med [dot] lu [dot] se

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00