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The Vascular Repair Process after Injury of the Carotid Artery is regulated by IL-1RI and MyD88 Signalling.

Author:
  • Amit Saxena
  • Uwe Rauch
  • Katarina Berg
  • Linda Andersson
  • Lisette Hollender
  • Ann-Margreth Carlsson
  • Maria Gomez
  • Anna Hultgårdh Nilsson
  • Jan Nilsson
  • Harry Björkbacka
Publishing year: 2011
Language: English
Pages: 350-357
Publication/Series: Cardiovascular Research
Volume: 91
Document type: Journal article
Publisher: Elsevier

Abstract english

Aim: The aim of this study was to determine if innate immune signalling influences the vascular repair process in response to mechanical injury of arteries in mice. Methods and Results: A non-obstructive collar was introduced around the carotid artery of MyD88-deficient mice and neointima formation was compared to that observed in MyD88-competent mice. MyD88-deficient mice are characterized by impaired signal transduction from interleukin (IL)-1/IL-18 receptors and most Toll-like receptors. The vascular response to injury was severely impaired in MyD88-deficient mice as neointima formation was not different from sham operated mice, whereas MyD88-competent mice displayed robust neointima formation. Furthermore, infiltration of CD68 positive leukocytes was dependent on MyD88. During the early response to injury, 3 days after collar placement, a transient increase in the expression of Toll-like receptor (TLR) 4 on vascular smooth muscle cells was observed. To determine the relative importance of IL-1 receptor and TLR4 activation in the vascular response to injury mice were injected with blocking antibodies to these receptors prior to the collar placement. Neointima formation was reduced by 80% in mice administered IL-1RI blocking antibodies compared to mice given a control antibody, whereas administration of TLR4 blocking antibodies was without effect. Conclusion: These results show that inhibition of MyD88- or IL-1 receptor signalling reduces neointima formation in response to vascular injury and could offer therapeutic options for reducing clinical complications of excessive smooth muscle cell proliferation, such as that observed in in-stent restenosis.

Keywords

  • Cardiac and Cardiovascular Systems

Other

Published
  • Vessel Wall Biology
  • Cardiovascular Research - Immunity and Atherosclerosis
  • ISSN: 1755-3245
E-mail: jan [dot] nilsson [at] med [dot] lu [dot] se

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