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Isabella Artner

Research team manager

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Islet Gene View-a tool to facilitate islet research

Author

  • Olof Asplund
  • Petter Storm
  • Vikash Chandra
  • Gad Hatem
  • Emilia Ottosson-Laakso
  • Dina Mansour-Aly
  • Ulrika Krus
  • Hazem Ibrahim
  • Emma Ahlqvist
  • Tiinamaija Tuomi
  • Erik Renström
  • Olle Korsgren
  • Nils Wierup
  • Mark Ibberson
  • Michele Solimena
  • Piero Marchetti
  • Claes Wollheim
  • Isabella Artner
  • Hindrik Mulder
  • Ola Hansson
  • Timo Otonkoski
  • Leif Groop
  • Rashmi B Prasad

Summary, in English

Characterization of gene expression in pancreatic islets and its alteration in type 2 diabetes (T2D) are vital in understanding islet function and T2D pathogenesis. We leveraged RNA sequencing and genome-wide genotyping in islets from 188 donors to create the Islet Gene View (IGW) platform to make this information easily accessible to the scientific community. Expression data were related to islet phenotypes, diabetes status, other islet-expressed genes, islet hormone-encoding genes and for expression in insulin target tissues. The IGW web application produces output graphs for a particular gene of interest. In IGW, 284 differentially expressed genes (DEGs) were identified in T2D donor islets compared with controls. Forty percent of DEGs showed cell-type enrichment and a large proportion significantly co-expressed with islet hormone-encoding genes; glucagon (GCG, 56%), amylin (IAPP, 52%), insulin (INS, 44%), and somatostatin (SST, 24%). Inhibition of two DEGs, UNC5D and SERPINE2, impaired glucose-stimulated insulin secretion and impacted cell survival in a human β-cell model. The exploratory use of IGW could help designing more comprehensive functional follow-up studies and serve to identify therapeutic targets in T2D.

Department/s

  • Genomics, Diabetes and Endocrinology
  • EXODIAB: Excellence of Diabetes Research in Sweden
  • MultiPark: Multidisciplinary research focused on Parkinson´s disease
  • StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
  • Developmental and Regenerative Neurobiology
  • Wallenberg Neuroscience Centre, Lund
  • Department Office of Clinical Sciences, Lund
  • CRC, Clinical Research Centre
  • Lund University Bioimaging Center
  • Diabetes - Islet Patophysiology
  • Neuroendocrine Cell Biology
  • Diabetic Complications
  • Endocrine Cell Differentiation and Function
  • Diabetes - Molecular Metabolism

Publishing year

2022

Language

English

Publication/Series

Life Science Alliance

Volume

5

Issue

12

Document type

Journal article

Publisher

Rockefeller University Press

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Genomics, Diabetes and Endocrinology
  • Developmental and Regenerative Neurobiology
  • LUDC (Lund University Diabetes Centre)
  • Diabetes - Islet Patophysiology
  • Neuroendocrine Cell Biology
  • Diabetic Complications
  • Endocrine Cell Differentiation and Function
  • Diabetes - Molecular Metabolism

ISBN/ISSN/Other

  • ISSN: 2575-1077