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ludc webb

Isabel Goncalves


ludc webb

Transthyretin expression in the postischemic brain


  • Daniela Talhada
  • Isabel Gonçalves
  • Cecília Reis Santos
  • Karsten Ruscher

Summary, in English

The unknown role of the carrier protein transthyretin (TTR) in mechanisms of functional recovery in the postischemic brain prompted us to study its expression following experimental stroke. Male C57/B6 mice (age 9 to 10 weeks) were subjected to permanent focal ischemia induced by photothrombosis (PT) and brain tissues were analyzed for ttr expression and TTR levels at 24 hours, 48 hours, 7 days and 14 days following the insult by RT-PCR, Western blot and immunohistochemistry. Fourteen days after PT, non-specific TTR-like immunoreactive globules were found in the ischemic core and surrounding peri-infarct region by immunohistochemistry that could not be allocated to DAPI positive cells. No TTR immunoreactivity was found when stainings were performed with markers for neurons (Neuronal Nuclei, NeuN), reactive astrocytes (glial fibrillary acidic protein, GFAP) or microglia (cluster of differentiation 68, CD68). In addition, we could not find TTR by immunoblotting in protein extracts obtained from the ischemic territory nor ttr expression by RT-PCR at all time points following PT. In all experiments, ttr expression in the choroid plexus and TTR in the mouse serum served as positive controls and recombinant legumain peptide as negative control. Together, our results indicate that TTR is not synthesized in brain resident cells in the ischemic infarct core and adjacent peri-infarct area. Thus, it seems unlikely that in situ synthesized TTR is involved in mechanisms of tissue reorganization during the first 14 days following PT.


  • LUBIN Lab- Lund Brain Injury laboratory for Neurosurgical research
  • MultiPark: Multidisciplinary research focused on Parkinson´s disease
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year










Document type

Journal article


Public Library of Science (PLoS)


  • Neurosciences



Research group

  • LUBIN Lab- Lund Brain Injury laboratory for Neurosurgical research


  • ISSN: 1932-6203