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Hindrik Mulder

Hindrik Mulder

Principal investigator

Hindrik Mulder

Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate.

Author

  • Helén Nilsson
  • David Lindgren
  • A Mandahl Forsberg
  • Hindrik Mulder
  • Håkan Axelson
  • Martin Johansson

Summary, in English

Changes of cellular metabolism are an integral property of the malignant potential of most cancer cells. Already in the 1930s, Otto Warburg observed that tumor cells preferably utilize glycolysis and lactate fermentation for energy production, rather than the mitochondrial oxidative phosphorylation dominating in normal cells, a phenomenon today known as the Warburg effect. Even though many tumor types display a high degree of aerobic glycolysis, they still retain the activity of other energy-producing metabolic pathways. One exception seems to be the clear cell variant of renal cell carcinoma, ccRCC, where the activity of most other pathways than that of glycolysis has been shown to be reduced. This makes ccRCC a promising candidate for the use of glycolytic inhibitors in treatment of the disease. However, few studies have so far addressed this issue. In this report, we show a strikingly reduced mitochondrial respiratory capacity of primary human ccRCC cells, resulting in enhanced sensitivity to glycolytic inhibition by 3-Bromopyruvate (3BrPA). This effect was largely absent in established ccRCC cell lines, a finding that highlights the importance of using biologically relevant models in the search for new candidate cancer therapies. 3BrPA markedly reduced ATP production in primary ccRCC cells, followed by cell death. Our data suggest that glycolytic inhibitors such as 3BrPA, that has been shown to be well tolerated in vivo, should be further analyzed for the possible development of selective treatment strategies for patients with ccRCC.

Department/s

  • EXODIAB: Excellence in Diabetes Research in Sweden
  • Division of Translational Cancer Research
  • Diabetes - Molecular Metabolism
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
  • Pathology, Malmö

Publishing year

2015

Language

English

Publication/Series

Cell Death & Disease

Volume

6

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Cell and Molecular Biology

Status

Published

Research group

  • Diabetes - Molecular Metabolism
  • Pathology, Malmö

ISBN/ISSN/Other

  • ISSN: 2041-4889