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Hindrik Mulder

Hindrik Mulder

Principal investigator

Hindrik Mulder

Hormone-sensitive lipase, the rate-limiting enzyme in triglyceride hydrolysis, is expressed and active in beta-cells

Author

  • Hindrik Mulder
  • Lena Stensson Holst
  • Håkan Svensson
  • Eva Degerman
  • Frank Sundler
  • Bo Ahrén
  • Patrik Rorsman
  • Cecilia Holm

Summary, in English

Triglycerides in the beta-cell may be important for stimulus-secretion coupling, through provision of a lipid-derived signal, and for pathogenetic events in NIDDM, where lipids may adversely affect beta-cell function. In adipose tissues, hormone-sensitive lipase (HSL) is rate-limiting in triglyceride hydrolysis. Here, we investigated whether this enzyme is also expressed and active in beta-cells. Northern blot analysis and reverse transcription-polymerase chain reaction demonstrated that HSL is expressed in rat islets and in the clonal beta-cell lines INS-1, RINm5F, and HIT-T15. Western blot analysis identified HSL in mouse and rat islets and the clonal beta-cells. In mouse and rat, immunocytochemistry showed a predominant occurrence of HSL in beta-cells, with a presumed cytoplasmic localization. Lipase activity in homogenates of the rodent islets and clonal beta-cells constituted 2.1 +/- 0.6% of that in adipocytes; this activity was immunoinhibited by use of antibodies to HSL. The established HSL expression and activity in beta-cells offer a mechanism whereby lipids are mobilized from intracellular stores. Because HSL in adipocytes is activated by cAMP-dependent protein kinase (PKA), PKA-regulated triglyceride hydrolysis in beta-cells may participate in the regulation of insulin secretion, possibly by providing a lipid-derived signal, e.g., long-chain acyl-CoA and diacylglycerol.

Department/s

  • Department of Experimental Medical Science
  • Insulin Signal Transduction
  • Medicine, Lund
  • Islet cell physiology
  • Molecular Endocrinology

Publishing year

1999

Language

English

Pages

228-232

Publication/Series

Diabetes

Volume

48

Issue

1

Document type

Journal article

Publisher

American Diabetes Association Inc.

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Insulin Signal Transduction
  • Islet cell physiology
  • Molecular Endocrinology

ISBN/ISSN/Other

  • ISSN: 1939-327X