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Hindrik Mulder

Hindrik Mulder

Principal investigator

Hindrik Mulder

Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase

Author

  • Lena Stenson
  • Dominique Langin
  • Hindrik Mulder
  • Henrik Laurell
  • Jacques Grober
  • Anders Bergh
  • Harvey W. Mohrenweiser
  • Gudrun Edgren
  • Cecilia Holm

Summary, in English

By catalyzing the rate-limiting step in adipose tissue lipolysis, hormone-sensitive lipase (HSL) is an important regulator of energy homeostasis. The role and importance of HSL in tissues other than adipose are poorly understood. We report here the cloning and expression of a testicular isoform, designated HSLtes. Due to an addition of amino acids at the NH2-termini, rat and human HSLtes consist of 1068 and 1076 amino acids, respectively, compared to the 768 and 775 amino acids, respectively, of the adipocyte isoform (HSLadi). A novel exon of 1.2 kb, encoding the human testis-specific amino acids, was isolated and mapped to the HSL gene, 16 kb upstream of the exons encoding HSLadi. The transcribed mRNA of 3.9 kb was specifically expressed in testis. No significant similarity with other known proteins was found for the testis-specific sequence. The amino acid composition differs from the HSLadi sequence, with a notable hydrophilic character and a high content of prolines and glutamines. COS cells, transfected by the 3.9-kb human testis cDNA, expressed a protein of the expected molecular mass (M(r) approximately 120,000) that exhibited catalytic activity similar to that of HSLadi. Immunocytochemistry localized HSL to elongating spermatids and spermatozoa; HSL was not detected in interstitial cells.

Department/s

  • Insulin Signal Transduction
  • Department of Experimental Medical Science

Publishing year

1996

Language

English

Pages

441-447

Publication/Series

Genomics

Volume

35

Issue

3

Document type

Journal article

Publisher

Academic Press

Topic

  • Endocrinology and Diabetes
  • Cancer and Oncology

Status

Published

Research group

  • Insulin Signal Transduction

ISBN/ISSN/Other

  • ISSN: 1089-8646