
Hindrik Mulder
Principal investigator

Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design
Author
Summary, in English
Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by validation with real samples. Here a GC/MS-based protocol using methoximation followed by silylation with N-tert-butyldi-methylsilyl-N-methyltrifluoroacetamide (MTBSTFA) for analysis of blood plasma metabolites is thoroughly developed and optimized from derivatization to detection with statistical design of experiments (DOE). Validation was performed with blood plasma samples and proved the methodology to be efficient, rapid and reliable with a total of 51 analyses performed in 24 h, with linear responses, low detection limits and good precision. The obtained chromatograms were much cleaner, due to the absence of glucose overloading, and the data was found to drift less with MTBSTFA derivatisation than with MTBSTFA derivatisation.
Department/s
- Diabetes - Molecular Metabolism
- EXODIAB: Excellence of Diabetes Research in Sweden
Publishing year
2012
Language
English
Pages
50-63
Publication/Series
Metabolomics
Volume
8
Issue
1
Document type
Journal article
Publisher
Springer
Topic
- Endocrinology and Diabetes
Keywords
- Metabolomics
- Gas chromatography
- Mass spectrometry
- MTBSTFA
- Blood
- plasma
- DOE
Status
Published
Research group
- Diabetes - Molecular Metabolism
ISBN/ISSN/Other
- ISSN: 1573-3882