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Hindrik Mulder

Hindrik Mulder

Principal investigator

Hindrik Mulder

Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design

Author

  • Anders Danielsson
  • Thomas Moritz
  • Hindrik Mulder
  • Peter Spégel

Summary, in English

Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by validation with real samples. Here a GC/MS-based protocol using methoximation followed by silylation with N-tert-butyldi-methylsilyl-N-methyltrifluoroacetamide (MTBSTFA) for analysis of blood plasma metabolites is thoroughly developed and optimized from derivatization to detection with statistical design of experiments (DOE). Validation was performed with blood plasma samples and proved the methodology to be efficient, rapid and reliable with a total of 51 analyses performed in 24 h, with linear responses, low detection limits and good precision. The obtained chromatograms were much cleaner, due to the absence of glucose overloading, and the data was found to drift less with MTBSTFA derivatisation than with MTBSTFA derivatisation.

Department/s

  • Diabetes - Molecular Metabolism
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2012

Language

English

Pages

50-63

Publication/Series

Metabolomics

Volume

8

Issue

1

Document type

Journal article

Publisher

Springer

Topic

  • Endocrinology and Diabetes

Keywords

  • Metabolomics
  • Gas chromatography
  • Mass spectrometry
  • MTBSTFA
  • Blood
  • plasma
  • DOE

Status

Published

Research group

  • Diabetes - Molecular Metabolism

ISBN/ISSN/Other

  • ISSN: 1573-3882