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ludc web

Gabriela de Paula

Postdoctoral fellow

ludc web

Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice


  • Henver Simionato Brunetta
  • Gabriela Cristina de Paula
  • Matheus Fritzen
  • Manuela Sozo Cecchini
  • Gustavo Jorge dos Santos
  • Evelise Maria Nazari
  • Alex Rafacho
  • Andreza Fabro de Bem
  • Everson Araújo Nunes

Summary, in English

Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in mitochondrial respiration, size, and complexity in the soleus skeletal muscle. We conclude that the positive effects of leucine on whole-body metabolism in DIO mice are associated with improvements in skeletal muscle mitochondrial function and morphology. Furthermore, leucine acute effects on mitochondrial respiration are mTORC1 dependent.

Publishing year





Journal of Functional Foods



Document type

Journal article




  • High-fat diet
  • Insulin resistance
  • Leucine
  • Mitochondria
  • Obesity




  • ISSN: 1756-4646