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Florentina Negoita

Visiting research fellow

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Salt-inducible kinase 2 regulates TFEB and is required for autophagic flux in adipocytes

Author

  • Florentina Negoita
  • Johanna Säll
  • Björn Morén
  • Karin Stenkula
  • Olga Göransson

Summary, in English

Dysregulation of autophagy has been observed in obesity and type 2 diabetes. Salt-inducible kinase 2 (SIK2), a member of the AMPK-related kinase family, is downregulated in adipocytes from obese or insulin resistant individuals and was previously demonstrated to regulate autophagy in cancer and normal cell lines. The aim of this study was thus to investigate a potential role of SIK2 in the regulation of adipocyte autophagy. To do so, SIK2 siRNA silencing or SIKs pharmacological inhibition of SIK2 was employed in murine differentiated 3T3-L1 adipocytes and autophagic flux was monitored. Our data indicate that SIK2 is required for both autophagic flux and expression of TFEB, the transcription factor that regulates autophagy, in adipocytes. The effect of SIK2 on autophagic flux occurs before the regulation of TFEB protein levels, suggesting different mechanisms whereby SIK2 stimulates autophagy. This study broadens the current knowledge on autophagy regulation and SIK2 function in adipocytes.

Department/s

  • EXODIAB: Excellence in Diabetes Research in Sweden
  • Protein Phosphorylation
  • Glucose Transport and Protein Trafficking

Publishing year

2019

Language

English

Pages

775-779

Publication/Series

Biochemical and Biophysical Research Communications

Volume

508

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Medicinal Chemistry

Keywords

  • Adipocytes
  • Autophagic flux
  • Autophagy
  • Salt-inducible kinase 2
  • TFEB

Status

Published

Research group

  • Protein Phosphorylation
  • Glucose Transport and Protein Trafficking

ISBN/ISSN/Other

  • ISSN: 0006-291X