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Filip Ottosson

Research student

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Dietary Data in the Malmö Offspring Study : Reproducibility, Method Comparison and Validation against Objective Biomarkers


  • Sophie Hellstrand
  • Filip Ottosson
  • Einar Smith
  • Louise Brunkwall
  • Stina Ramne
  • Emily Sonestedt
  • Peter M Nilsson
  • Olle Melander
  • Marju Orho-Melander
  • Ulrika Ericson

Summary, in English

Irregular dietary intakes impairs estimations from food records. Biomarkers and method combinations can be used to improve estimates. Our aim was to examine reproducibility from two assessment methods, compare them, and validate intakes against objective biomarkers. We used the Malmö Offspring Study (55% women, 18-71 y) with data from a 4-day food record (4DFR) and a short food frequency questionnaire (SFFQ) to compare (1) repeated intakes (n = 180), (2) intakes from 4DFR and SFFQ (n = 1601), and (3) intakes of fatty fish, fruits and vegetables, and citrus with plasma biomarkers (n = 1433) (3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid [CMPF], β-carotene and proline betaine). We also combined 4DFR and SFFQ estimates using principal component analysis (PCA). Moderate correlations were seen between repeated intakes (4DFR median ρ = 0.41, SFFQ median ρ = 0.59) although lower for specific 4DFR-items, especially fatty/lean fish (ρ ≤ 0.08). Between-method correlations (median ρ = 0.33) were higher for intakes of overall food groups compared to specific foods. PCA scores for citrus (proline betaine ρ = 0.53) and fruits and vegetables (β-carotene: ρ = 0.39) showed the highest biomarker correlations, whereas fatty fish intake from the SFFQ per se showed the highest correlation with CMPF (ρ = 0.46). To conclude, the reproducibility of SFFQ data was superior to 4DFR data regarding irregularly consumed foods. Method combination could slightly improve fruit and vegetable estimates, whereas SFFQ data gave most valid fatty fish intake.


  • Diabetes - Cardiovascular Disease
  • EpiHealth: Epidemiology for Health
  • EXODIAB: Excellence of Diabetes Research in Sweden
  • Department of Clinical Sciences, Malmö
  • Cardiovascular Research - Hypertension
  • Nutrition Epidemiology
  • History of Medicine
  • Internal Medicine - Epidemiology

Publishing year










Document type

Journal article




  • Nutrition and Dietetics


  • food intake
  • dietary assessment method
  • reproducibility
  • validation
  • biomarker
  • fish
  • vegetables
  • fruits
  • citrus



Research group

  • Diabetes - Cardiovascular Disease
  • Cardiovascular Research - Hypertension
  • Nutrition Epidemiology
  • Internal Medicine - Epidemiology


  • ISSN: 2072-6643