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Eva Degerman

Eva Degerman

Professor

Eva Degerman

Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B.

Author

  • Rebecka Lindh
  • Faiyaz Ahmad
  • Svante Resjö
  • Peter James
  • Jeong S Yang
  • Henry M Fales
  • Vincent Manganiello
  • Eva Degerman

Summary, in English

Phosphodiesterase 3B (PDE3B) is an important component of insulin and cAMP-dependent signalling pathways. In order to study phosphorylation of PDE3B, we have used an adenoviral system to express recombinant flag-tagged PDE3B in primary rat adipocytes and H4IIE hepatoma cells. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. We found that PDE3B is multisite phosphorylated in adipocytes and H4IIE hepatoma cells in response to all these stimuli. Several sites were identified; serine (S)273, S296, S421, S424/5, S474 and S536 were phosphorylated in adipocyte as well as H4IIE hepatoma cells whereas S277 and S507 were phosphorylated in hepatoma cells only. Several of the sites were phosphorylated by insulin as well as cAMP-increasing hormones indicating integration of the two signalling pathways upstream of PDE3B, maybe at the level of protein kinase B.

Department/s

  • Insulin Signal Transduction
  • Department of Immunotechnology

Publishing year

2007

Language

English

Pages

584-592

Publication/Series

Biochimica et Biophysica Acta: Molecular Cell Research

Volume

1773

Issue

4

Document type

Journal article

Publisher

Elsevier

Topic

  • Endocrinology and Diabetes

Keywords

  • Phosphorylation
  • PDE3B
  • Adipocyte
  • Insulin
  • Hepatocyte
  • cAMP

Status

Published

Research group

  • Insulin Signal Transduction

ISBN/ISSN/Other

  • ISSN: 0167-4889