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Eva Degerman

Eva Degerman

Professor

Eva Degerman

Insulin induced phosphorylation and activation of the cGMP-inhibited cAMP phosphodiesterase in human platelets

Author

  • Pilar Lopez-Aparicio
  • Ana Rascon
  • Vincent C Manganiello
  • Karl-Erik Andersson
  • Per Belfrage
  • Eva Degerman

Summary, in English

Insulin induced phosphorylation and activation of the cGMP inhibited cAMP phosphodiesterase (cGI-PDE) in human platelets were demonstrated after isolation of the enzyme with specific polyclonal cGI-PDE antibodies. The demonstration of this insulin effect required suppression of basal cGI-PDE phosphorylation, through the use of the protein kinase inhibitor H-7 (1-(5-isoquinolinylsulfonyl)-2-methylpiperazine). The human platelet insulin receptor beta-subunit, previously identified as a 97 kDa polypeptide, was detected with the use of wheat germ agglutinin chromatography and anti-phosphotyrosine antibodies. These results suggest that insulin, through phosphorylation/activation of cGI-PDE, could decrease cAMP/cAMP dependent protein kinase (cAMP-PK) activity and thereby make the platelets more sensitive towards aggregating agents.

Department/s

  • Biotechnology
  • Division of Clinical Chemistry and Pharmacology
  • Insulin Signal Transduction

Publishing year

1992

Language

English

Pages

517-523

Publication/Series

Biochemical and Biophysical Research Communications

Volume

186

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Status

Published

Research group

  • Insulin Signal Transduction

ISBN/ISSN/Other

  • ISSN: 1090-2104