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Protein kinase B is expressed in pancreatic beta cells and activated upon stimulation with insulin-like growth factor I

Author:
  • Lena Stenson
  • Hindrik Mulder
  • V Manganiello
  • Frank Sundler
  • Bo Ahrén
  • Cecilia Holm
  • Eva Degerman
Publishing year: 1998
Language: English
Pages: 181-186
Publication/Series: Biochemical and Biophysical Research Communications
Volume: 250
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

Protein kinase B (PKB) is involved in signaling to a multitude of important cellular events and is activated by insulin and growth factors, including insulin-like growth factor I (IGF-I). We show here expression of PKB in pancreatic islets and in the beta cell lines HIT-T15, INS-1, and RINm5F. Expression of PKB mRNA and the presence of PKB isoforms (alpha, beta, and gamma) were assessed by Northern blot analysis and RT-PCR, respectively. Antibodies recognizing different parts of PKB isoforms were employed to demonstrate PKB protein expression by immunoblot analysis. By use of immunohistochemistry in rat and mouse pancreatic tissue sections, PKB was localized to predominantly beta cells. Regulation of PKB was examined in INS-1 and RINm5F cells; upon stimulation with IGF-I (5-10 min), PKB was phosphorylated and activated (approximately 3-fold) by a wortmannin-sensitive mechanism, indicating involvement of phosphatidylinositol-3 kinase. The possible participation of PKB in signal transduction pathways modulating cAMP-dependent insulin secretion and in proliferation of beta cells is discussed.

Keywords

  • Biological Sciences

Other

Published
  • Insulin Signal Transduction
  • Molecular Endocrinology
  • ISSN: 1090-2104
Eva Degerman
E-mail: eva [dot] degerman [at] med [dot] lu [dot] se

Professor

Insulin Signal Transduction

+46 46 222 85 83

+46 70 885 83 62

BMC C1121b

66

Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00