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Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.

Author:
  • Emilia Heimann
  • Helena Jones
  • Svante Resjö
  • Vincent C Manganiello
  • Lena Stenson
  • Eva Degerman
Publishing year: 2010
Language: English
Publication/Series: PLoS ONE
Volume: 5
Issue: 12
Document type: Journal article
Publisher: Public Library of Science

Abstract english

As shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme. Here, we show that PDE3B is activated in response to high glucose, insulin and cAMP elevation in rat pancreatic islets and INS-1 (832/13) cells. Activation by glucose was not affected by the presence of diazoxide. PDE3B activation was coupled to an increase as well as a decrease in total phosphorylation of the enzyme. In addition to PDE3B, several other PDEs were detected in human pancreatic islets: PDE1, PDE3, PDE4C, PDE7A, PDE8A and PDE10A. We conclude that PDE3B is activated in response to agents relevant for β-cell function and that activation is linked to increased as well as decreased phosphorylation of the enzyme. Moreover, we conclude that several PDEs are present in human pancreatic islets.

Keywords

  • Endocrinology and Diabetes

Other

Published
  • Insulin Signal Transduction
  • Molecular Endocrinology
  • ISSN: 1932-6203
Eva Degerman
E-mail: eva [dot] degerman [at] med [dot] lu [dot] se

Professor

Insulin Signal Transduction

+46 46 222 85 83

+46 70 885 83 62

BMC C1121b

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