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Erik Renström

Erik Renström

Vice-chancellor

Erik Renström

Redox control of exocytosis - Regulatory role of NADPH, thioredoxin, and glutaredoxin

Author

  • Rosita Ivarsson
  • R Quintens
  • S Dejonghe
  • K Tsukamoto
  • P Veld
  • Erik Renström
  • FC Schuit

Summary, in English

Cellular redox state is an important metabolic variable, influencing many aspects of cell function like growth, apoptosis, and reductive biosynthesis. In this report, we identify NADPH as a candidate signaling molecule for exocytosis in neuroendocrine cells. In pancreatic beta-cells, glucose acutely raised the NADPH-to-NADP(+) ratio and stimulated insulin release in parallel. Furthermore, intracellular addition of NADPH directly stimulated exocytosis of insulin granules. Effects of NADPH on exocytosis are proposed to be mediated by the redox proteins glutaredoxin (GRX) and thioredoxin (TRX) on the basis of the following evidence: 1) Expression of GRX mRNA is very high in beta-cells compared with other studied tissues, and GRX protein expression is high in islets and in brain; 2) GRX and TRX are localized in distinct microdomains in the cytosol of beta-cells; and 3) microinjection of recombinant GRX potentiated effects of NADPH on exocytosis, whereas TRX antagonized the NADPH effect. We propose that the NADPEVGRX/ TRX redox regulation mediates a novel signaling pathway of nutrient-induced insulin secretion.

Department/s

  • Diabetes - Islet Patophysiology

Publishing year

2005

Language

English

Pages

2132-2142

Publication/Series

Diabetes

Volume

54

Issue

7

Document type

Journal article

Publisher

American Diabetes Association Inc.

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Diabetes - Islet Patophysiology

ISBN/ISSN/Other

  • ISSN: 1939-327X