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Erik Renström

Erik Renström

Vice-chancellor

Erik Renström

Myosin 5a controls insulin granule recruitment during late-phase secretion.

Author

  • Rosita Ivarsson
  • Xingjun Jing
  • Laurent Waselle
  • Romano Regazzi
  • Erik Renström

Summary, in English

We have examined the importance of the actin-based molecular motor myosin 5a for insulin granule transport and insulin secretion. Expression of myosin 5a was downregulated in clonal INS-1E cells using RNAinterference. Stimulated hormone secretion was reduced by 46% and single-cell exocytosis, measured by capacitance recordings, was inhibited by 42% after silencing. Silencing of Slac-2c/MYRIP, which links insulin granules to myosin 5a, resulted in similar inhibition of single-cell exocytosis. Antibody inhibition of the myosin 5a-Slac-2c/MYRIP interaction significantly reduced the recruitment of insulin granules for release. The pool of releasable granules independent of myosin 5a activity was estimated to approximately 550 granules. Total internal reflection microscopy was then applied to directly investigate granule recruitment to the plasma membrane. Silencing of myosin 5a inhibited granule recruitment during late phase of insulin secretion. In conclusion, we propose a model where insulin granules are transported through the actin network via both myosin 5a-mediated transport and via passive diffusion, with the former playing the major role during stimulatory conditions.

Department/s

  • Diabetes - Islet Patophysiology
  • Neurosurgery

Publishing year

2005

Language

English

Pages

1027-1035

Publication/Series

Traffic: the International Journal of Intracellular Transport

Volume

6

Issue

11

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Basic Medicine

Status

Published

Research group

  • Diabetes - Islet Patophysiology

ISBN/ISSN/Other

  • ISSN: 1398-9219