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Erik Renström

Erik Renström

Vice-chancellor

Erik Renström

Neurotransmitter-induced inhibition of exocytosis in insulin-secreting beta cells by activation of calcineurin

Author

  • Erik Renström
  • Wei-Guang Ding
  • Krister Bokvist
  • Patrik Rorsman

Summary, in English

Neurotransmitters and hormones such as somatostatin, galanin, and adrenalin reduce insulin secretion. Their inhibitory action involves direct interference with the exocytotic machinery. We have examined the molecular processes underlying this effect using high resolution measurements of cell capacitance. Suppression of exocytosis was maximal at concentrations that did not cause complete inhibition of glucose-stimulated electrical activity. This action was dependent on activation of G proteins but was not associated with inhibition of the voltage-dependent Ca2+ currents or adenylate cyclase activity. The molecular processes initiated by the agonists culminate in the activation of the Ca(2+)-dependent protein phosphatase calcineurin, and suppression of the activity of this enzyme abolishes their action on exocytosis. We propose that mechanisms similar to those we report here may contribute to adrenergic and peptidergic inhibition of secretion in other neuroendocrine cells and in nerve terminals.

Publishing year

1996

Language

English

Pages

513-522

Publication/Series

Neuron

Volume

17

Issue

3

Document type

Journal article

Publisher

Cell Press

Topic

  • Neurosciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 0896-6273