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Enming Zhang

Research team manager

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The effects of embryonic bodies on differentiation of mouse embryonic stem cells to neuronal cells in vitro

Author

  • Shufang Zhang
  • Enming Zhang
  • Xiaoxiang Zheng

Summary, in English

This study was directed at assessing the effects of embryonic bodies on differentiation of the embryonic stem cells. The embryonic bodies of different days gained by means of hanging, suspending and hung-suspended were processed by all-trans retinotic acids for 4 days. Then they were detected by immunocytochemistry methods and were measured to detect the fluorescence intensity changes of calcium after Glu stimulation. During this period, we observed the differentiation process and compared the differentiation ratio of embryonic stem cells processed by means of hanging, suspending for several days. It was found that the differentiation ratio of EBs obtained by suspending for 3 days, or by hanging for 3 days and suspending for 1 day, is relatively higher, which can help us find out the intrinsic differentiation mechanism of embryonic stem cells.

Publishing year

2006-06

Language

English

Pages

620-624

Publication/Series

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi

Volume

23

Issue

3

Document type

Journal article

Publisher

Sichuan Society for Biomedical Engineering

Topic

  • Cell and Molecular Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1001-5515