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Enming Zhang

Research team manager

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Cell cycle synchronization of embryonic stem cells : Effect of serum deprivation on the differentiation of embryonic bodies in vitro

Author

  • Enming Zhang
  • Xiaolong Li
  • Shufang Zhang
  • Liangqiang Chen
  • Xiaoxiang Zheng

Summary, in English

Research on stem-cell transplantation has indicated that the success of transplantation largely depends on synchronizing donor cells into the G0/G1 phase. In this study, we investigated the profile of embryonic stem (ES) cell synchronization and its effect on the formation of embryonic bodies (EBs) using cell culture with serum deprivation. The D3 cell line of ES cells was used, and parameters such as cell proliferation and activity, EB formation, and expression of stage-specific embryonic antigen-1 and Oct-4 were investigated. Results showed that the percentage of G0/G1 stage in serum deprivation culture is significantly higher than that in culture with serum supplementation. Synchronized ES cells can reenter the normal cell cycle successfully after serum supply. EBs formed from synchronized ES cells have higher totipotency capability to differentiate into functional neuronal cells than EBs formed from unsynchronized ES cells. Our study provides a method for ES treatment before cell transplantation that possibly helps to decrease the rate of cell death after transplantation.

Publishing year

2005-08

Language

English

Pages

1171-1177

Publication/Series

Biochemical and Biophysical Research Communications

Volume

333

Issue

4

Document type

Journal article

Publisher

Elsevier

Topic

  • Cell and Molecular Biology

Keywords

  • Cell synchronization
  • Differentiation
  • Embryonic bodies
  • Embryonic stem cells
  • Serum deprivation

Status

Published

ISBN/ISSN/Other

  • ISSN: 0006-291X