
David Nicholls
Visiting research fellow
David Nicholls is one of three senior researchers in within LUDC. Davids main research base at the Buck Institute for Research on Aging, Novato (near San Fransisco). David was one of the founding Faculty there and since 2008 have continued on a part-time basis, spending 3-4 months a year at the bench developing techniques for investigating mitochondrial bioenergetics in intact cells. You can find out more about David on the Buck website http://www.buckinstitute.org/nichollsLab.
For the remaining part of the year David is based in Lund and have a part-time position with the LUDC. Davids career has been devoted to mitochondrial bioenergetics, with an emphasis at different times on chemiosmotic coupling of ATP synthesis (David co-author the standard textbook on the subject and is currently writing the 4th edition), brown fat metabolism (uncoupling protein 1), calcium transport, exocytosis from synaptosomes (isolated nerve terminals), neuronal bioenergetics and most recently mitochondrial control of plasma membrane potential and clacium channels in insulinoma cell lines.
This last David is investigating at the Buck and in LUDC in collaboration with Hindrik´s group.
David has helped to establish the Seahorse technology in LUDC to monitor cell function, together with a range of live-cell confocal fluorescent imaging techniques (plasma membrane potential, mitochondrial membrane potential, cytoplasmic Ca2+ etc).
David also help to run bioenergetic teaching courses around the world.
David is happy to help and collaborate with any group within the LUDC. Feel free to contact his using the Buch email
dnicholls [at] buckinstitute [dot] org
Publications
Displaying of publications. Sorted by year, then title.
Mitochondrial clearance of calcium facilitated by MICU2 controls insulin secretion
N. Vishnu, A. Hamilton, A. Bagge, A. Wernersson, E. Cowan, et al.
(2021) Molecular Metabolism, 51
Journal articleThe pathogenetic role of β-cell mitochondria in type 2 diabetes
Malin Fex, Lisa M. Nicholas, Neelanjan Vishnu, Anya Medina, Vladimir V. Sharoyko, et al.
(2018) Journal of Endocrinology, 236 p.145-149
Journal article reviewThe pancreatic β-cell : A bioenergetic perspective
David G. Nicholls
(2016) Physiological Reviews, 96 p.1385-1447
Journal articleCharacterization of Stimulus-Secretion Coupling in the Human Pancreatic EndoC-βH1 Beta Cell Line.
Lotta Andersson, Berengere Valtat, Annika Bagge, Vladimir Sharoyko, David Nicholls, et al.
(2015) PLoS ONE, 10
Journal articleChronic high glucose and pyruvate levels differentially affect mitochondrial bioenergetics and fuel-stimulated insulin secretion from clonal INS-1 832/13 cells.
Isabel Göhring, Vladimir Sharoyko, Siri Malmgren, Lotta Andersson, Peter Spégel, et al.
(2014) Journal of Biological Chemistry, 289 p.3786-3798
Journal articleUse of potentiometric fluorophores in the measurement of mitochondrial reactive oxygen species.
Brian M Polster, David Nicholls, Shealinna X Ge, Brian A Roelofs
(2014) Methods in Enzymology, 547 p.225-250
Journal articlePlasma Membrane Potential Oscillations in Insulin Secreting Ins-1 832/13 Cells do not Require Glycolysis and are not Initiated by Fluctuations in Mitochondrial Bioenergetics.
Isabel Goehring, Akos A Gerencser, Sara Schmidt, Martin D Brand, Hindrik Mulder, et al.
(2012) Journal of Biological Chemistry, 287 p.15706-15717
Journal articleA lipotoxicity model in the INS-1 832/13 beta cell line and the epigenetic alterations induced by it
Siri Malmgren, Vladimir Sharoyko, David Nicholls, Cecilia Nagorny, Peter Spégel, et al.
(2010) Diabetologia, 53 p.348-348
Conference paper: abstractThe membrane potential response is altered in pancreatic beta cells chronically exposed to high glucose
Isabel Göhring, Thomas Köck, Hindrik Mulder, David Nicholls
(2010) Diabetologia, 53 p.554-554
Conference paper: abstractStochastic aspects of transmitter release and bioenergetic dysfunction in isolated nerve terminals
David Nicholls
(2010) Biochemical Society Transactions, 38 p.457-459
Conference paperMitochondrial ion circuits.
David Nicholls
(2010) Essays in Biochemistry, 47 p.25-35
Journal articleMitochondrial spare respiratory capacity and the life and death of neurons
David Nicholls
(2009) The FEBS Journal, 276 p.35-35
Conference paper: abstractSpare respiratory capacity, oxidative stress and excitotoxicity
David Nicholls
(2009) Biochemical Society Transactions, 37 p.1385-1388
Journal articleDifferences in metabolic regulation between glucose responsive INS-1 832/13 and its glucose non-responsive INS-1 832/2 sister clonal rat beta cell line decides their beta cell functionality
Siri Malmgren, Vladimir Sharoyko, David Nicholls, Hindrik Mulder
(2009) Diabetologia, 52 p.454-454
Conference paper: abstractTight coupling between glucose and mitochondrial metabolism in clonal beta-cells is required for robust insulin secretion.
Siri Malmgren, David Nicholls, Jalal Taneera, Karl Bacos, Thomas Koeck, et al.
(2009) Journal of Biological Chemistry, 284 p.32395-32404
Journal article