Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Corrado Cilio

Professor

Default user image.

Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.

Author

  • Caroline Bolmeson
  • Jonathan Esguerra
  • S Albert Salehi
  • Dina Speidel
  • Lena Eliasson
  • Corrado Cilio

Summary, in English

Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.

Department/s

  • Create Health
  • Evolutionary ecology
  • Diabetes - Islet Cell Exocytosis
  • Islet cell physiology
  • Diabetes - Immunovirology
  • EXODIAB: Excellence in Diabetes Research in Sweden

Publishing year

2011

Language

English

Pages

16-22

Publication/Series

Biochemical and Biophysical Research Communications

Volume

Dec

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis
  • Islet cell physiology
  • Diabetes - Immunovirology

ISBN/ISSN/Other

  • ISSN: 1090-2104