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Corrado Cilio

Professor

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Evidence for De Novo Expression of Thymic Insulin by Peripheral Bone Marrow-derived Cells.

Author

  • Maria Carlsén
  • Corrado Cilio

Summary, in English

Thymic expression of insulin has been suggested to play a major role in negative selection of autoreactive T cells and tolerance induction against pancreatic beta cells. Furthermore, the expression of insulin in peripheral antigen-presenting cells (APC) has been clearly demonstrated but whether thymic negative selection and tolerance induction also depends on peripheral influx of self-antigens (Ag) remains to be conclusively demonstrated. In this study, we wanted to test whether peripheral influx of insulin expressing cells might contribute to negative selection. In order to address this question, we used mice deficient in the Ins1 and Ins2 genes. Embryonic thymi either deficient in both insulin genes or expressing Ins2 were dissected and transplanted under the kidney capsule of athymic nude mice recipients. After indicated time points, grafted thymi were removed and analysed for insulin re-expression and for the emergence of autoreactive T cells. The analysis revealed a re-expression of Ins2 in grafted insulin deficient thymi suggesting that self-Ag expression in the thymus is not only intrinsically regulated but peripheral influx of APC capable of expressing insulin might contribute to thymic selection and tolerance induction.

Department/s

  • Diabetes - Immunovirology

Publishing year

2008

Language

English

Pages

67-74

Publication/Series

Scandinavian Journal of Immunology

Volume

68

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Immunology in the medical area

Status

Published

Research group

  • Diabetes - Immunovirology

ISBN/ISSN/Other

  • ISSN: 1365-3083