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Cecilia Holm

Professor

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Perilipin 1 binds to aquaporin 7 in human adipocytes and controls its mobility via protein kinase A mediated phosphorylation

Author

  • Jesper Söndergaard Hansen
  • Christian Krintel
  • Malin Hernebring
  • Tatu J K Haataja
  • Sofia de Marè
  • Sebastian Wasserstrom
  • Urszula Kosinska-Eriksson
  • Madelene Palmgren
  • Cecilia Holm
  • Karin G. Stenkula
  • Helena A. Jones
  • Karin Lindkvist-Petersson

Summary, in English

Accumulating evidence suggests that dysregulated glycerol metabolism contributes to the pathophysiology of obesity and type 2 diabetes. Glycerol efflux from adipocytes is regulated by the aquaglyceroporin AQP7, which is translocated upon hormone stimulation. Here, we propose a molecular mechanism where the AQP7 mobility in adipocytes is dependent on perilipin 1 and protein kinase A. Biochemical analyses combined with ex vivo studies in human primary adipocytes, demonstrate that perilipin 1 binds to AQP7, and that catecholamine activated protein kinase A phosphorylates the N-terminus of AQP7, thereby reducing complex formation. Together, these findings are indicative of how glycerol release is controlled in adipocytes, and may pave the way for the future design of drugs against human metabolic pathologies.

Department/s

  • EXODIAB: Excellence in Diabetes Research in Sweden
  • Medical Structural Biology
  • Medical Protein Science
  • Glucose Transport and Protein Trafficking
  • Molecular Endocrinology
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation

Publishing year

2016-12-01

Language

English

Pages

1731-1742

Publication/Series

Metabolism, Clinical and Experimental

Volume

65

Issue

12

Document type

Journal article

Publisher

Elsevier

Topic

  • Cell and Molecular Biology

Keywords

  • Adipocyte
  • Aquaporin 7
  • Glycerol
  • Perilipin 1
  • PKA

Status

Published

Research group

  • Medical Structural Biology
  • Medical Protein Science
  • Glucose Transport and Protein Trafficking
  • Molecular Endocrinology

ISBN/ISSN/Other

  • ISSN: 0026-0495