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Cecilia Holm

Professor

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Characterization of a novel testicular form of human hormone-sensitive lipase

Author

  • A Mairal
  • N Melaine
  • H Laurell
  • J Grober
  • Lena Stenson
  • T Guillaudeux
  • Cecilia Holm
  • B Jegou
  • D Langin

Summary, in English

Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.

Department/s

  • Insulin Signal Transduction
  • Molecular Endocrinology

Publishing year

2002

Language

English

Pages

286-290

Publication/Series

Biochemical and Biophysical Research Communications

Volume

291

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • testis
  • spermatogenesis
  • lipid metabolism
  • fertility
  • lipase
  • esterase

Status

Published

Research group

  • Insulin Signal Transduction
  • Molecular Endocrinology

ISBN/ISSN/Other

  • ISSN: 1090-2104