Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Cecilia Holm

Professor

Default user image.

Effect of murine strain on metabolic pathways of glucose production after brief or prolonged fasting

Author

  • SC Burgess
  • FMH Jeffrey
  • C Storey
  • A Milde
  • N Hausler
  • ME Merritt
  • Hindrik Mulder
  • Cecilia Holm
  • AD Sherry
  • CR Malloy

Summary, in English

Background strain is known to influence the way a genetic manipulation affects mouse phenotypes. Despite data that demonstrate variations in the primary phenotype of basic inbred strains of mice, there is limited data available about specific metabolic fluxes in vivo that may be responsible for the differences in strain phenotypes. In this study, a simple stable isotope tracer/NMR spectroscopic protocol has been used to compare metabolic fluxes in ICR, FVB/N (FVB), C57BL/6J (B6), and 129S1/SvImJ (129) mouse strains. After a short-term fast in these mice, there were no detectable differences in the pathway fluxes that contribute to glucose synthesis. However, after a 24-h fast, B6 mice retain some residual glycogenolysis compared with other strains. FVB mice also had a 30% higher in vivo phosphoenolpyruvate carboxykinase flux and total glucose production from the level of the TCA cycle compared with B6 and 129 strains, while total body glucose production in the 129 strain was similar to 30% lower than in either FVB or B6 mice. These data indicate that there are inherent differences in several pathways involving glucose metabolism of inbred strains of mice that may contribute to a phenotype after genetic manipulation in these animals. The techniques used here are amenable to use as a secondary or tertiary tool for studying mouse models with disruptions of intermediary metabolism.

Department/s

  • Diabetes - Molecular Metabolism
  • Molecular Endocrinology

Publishing year

2005

Language

English

Pages

53-61

Publication/Series

American Journal of Physiology: Endocrinology and Metabolism

Volume

289

Issue

1

Document type

Journal article

Publisher

American Physiological Society

Topic

  • Physiology

Keywords

  • stable isotope
  • metabolic flux
  • phosphoenolpyruvate carboxykinase
  • nuclear magnetic resonance
  • tricarboxylic acid cycle
  • tracers
  • deuterium
  • mouse phenotype

Status

Published

Research group

  • Diabetes - Molecular Metabolism
  • Molecular Endocrinology

ISBN/ISSN/Other

  • ISSN: 1522-1555