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Cecilia Holm

Professor

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Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa

Author

  • J Grober
  • S Lucas
  • Maria Sörhede Winzell
  • I Zaghini
  • A Mairal
  • Juan Antonio Contreras
  • P Besnard
  • Cecilia Holm
  • D Langin

Summary, in English

The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol lipase activity was unchanged in the duodenum and reduced in jejunum. Neutral cholesterol esterase activity was totally abolished in duodenum, jejunum, and ileum of HSL-null mice. Analysis of HSL mRNA structure showed two types of transcripts expressed in equal amounts with alternative 5'-ends transcribed from two exons. This work demonstrates that HSL is expressed in the mucosa of the small intestine. The results also reveal that the enzyme participates in acylglycerol hydrolysis in jejunal enterocytes and cholesteryl ester hydrolysis throughout the small intestine.

Department/s

  • Medicine, Lund
  • Department of Experimental Medical Science
  • Molecular Endocrinology

Publishing year

2003

Language

English

Pages

6510-6515

Publication/Series

Journal of Biological Chemistry

Volume

278

Issue

8

Document type

Journal article

Publisher

ASBMB

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Molecular Endocrinology

ISBN/ISSN/Other

  • ISSN: 1083-351X