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Carina Törn

Coordinator

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C-peptide in dried blood spots.

Author

  • Jesper Johansson
  • Charlotte Becker
  • Nils Persson
  • Malin Fex
  • Carina Törn

Summary, in English

Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log S-C-peptide at baseline was 0 and limits of agreements were -0.18 to +0.18. Mean difference between converted log DBS-C-peptide values after six months and log S-C-peptide value from baseline was -0.01 and limits of agreement were -0.20-0.19. The lowest value detected with the DBS-technique corresponded to serum C-peptide 0.44 nmol/L. We concluded that DBS-C-peptide can be used as a first line screening test to monitor normal beta cell function. C-peptide on filters remained stable for six months.

Department/s

  • Clinical Chemistry, Malmö
  • Celiac Disease and Diabetes Unit
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2010

Language

English

Pages

404-409

Publication/Series

Scandinavian Journal of Clinical and Laboratory Investigation

Volume

70

Document type

Journal article

Publisher

Informa Healthcare

Topic

  • Medicinal Chemistry
  • Endocrinology and Diabetes

Status

Published

Research group

  • Clinical Chemistry, Malmö
  • Diabetes and Celiac Unit

ISBN/ISSN/Other

  • ISSN: 1502-7686