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Ashfaq Ali

Bioinformatician

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Effector-driven marker development and cloning of resistance genes against Phytophthora infestans in potato breeding clone SW93-1015

Author

  • Marit Lenman
  • Ashfaq Ali
  • Per Mühlenbock
  • Ulrika Carlson-Nilsson
  • Erland Liljeroth
  • Nicolas Champouret
  • Vivianne G A A Vleeshouwers
  • Erik Andreasson

Summary, in English

KEY MESSAGE: We show the usefulness of integrating effector screening in a breeding program and in resistance gene cloning, with Phytophthora resistance in the Swedish potato breeding clone SW93-1015 as an example. Phytophthora infestans is one of the most devastating plant pathogens worldwide. We have earlier found that the SW93-1015 potato breeding clone has an efficient resistance against P. infestans under field conditions in Sweden, which has an unusually high local diversity of the pathogen. This potato clone has characteristics that are different from classical R-gene-mediated resistance such as elevated levels of hydrogen peroxide (H2O2) under controlled conditions. Analysis of 76 F1 potato progenies from two individual crosses resulted in nearly 50% resistant clones, from both crosses. This result suggests that the SW93-1015 clone has a simplex genotype for this trait. Screening with over 50 different P. infestans effectors, containing the conserved motif RXLR (for Arg, any amino acid, Leu, Arg), revealed a specific response to Avr2, which suggests that SW93-1015 might contain a functional homolog of the R2 resistance gene. We cloned eight R2 gene homologs from SW93-1015, whereof seven have not been described before and one gene encoded a protein identical to Rpi-ABPT. Expression of this gene in potato cultivar Désirée provided R2-specific resistance, whereas other homologues did not. Using RNAseq analyses we designed a new DNA marker for the R2 resistance in SW93-1015. In summary, we have demonstrated the use of effector screening in practical breeding material and revealed the key resistance mechanism for SW93-1015.

Publishing year

2016-01

Language

English

Pages

15-105

Publication/Series

Theoretical And Applied Genetics

Volume

129

Issue

1

Document type

Journal article

Publisher

Springer

Topic

  • Genetics and Breeding

Keywords

  • Amino Acid Sequence
  • Breeding
  • Cloning, Molecular
  • Disease Resistance/genetics
  • Genes, Plant
  • Genetic Markers
  • Genotype
  • Molecular Sequence Data
  • Phytophthora infestans
  • Plant Diseases/genetics
  • Plants, Genetically Modified/genetics
  • Solanum tuberosum/genetics

Status

Published

ISBN/ISSN/Other

  • ISSN: 0040-5752