
Ashfaq Ali
Bioinformatician

Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes
Author
Summary, in English
Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.
Department/s
- Diabetes - Epigenetics
- EXODIAB: Excellence of Diabetes Research in Sweden
Publishing year
2016-12-01
Language
English
Pages
1601-1617
Publication/Series
Epigenomics
Volume
8
Issue
12
Document type
Journal article
Publisher
Future Medicine Ltd.
Topic
- Endocrinology and Diabetes
- Medical Genetics
Keywords
- adipogenesis
- adiponectin
- DNA methylation
- epigenetics
- stem cells
Status
Published
Research group
- Diabetes - Epigenetics
ISBN/ISSN/Other
- ISSN: 1750-1911