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Åsalinda Lethagen

Physician

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GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination

Author

  • L. M. Bekris
  • R. A. Jensen
  • E. Lagerquist
  • T. R. Hall
  • Carl-David Agardh
  • Corrado Cilio
  • ÅsaLinda Lethagen
  • Åke Lernmark
  • J. A. Robertson
  • C. S. Hampe

Summary, in English

Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65. Conclusions Our data suggest that the apparent beneficial effects of 20 mu g alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern.

Department/s

  • Department of Clinical Sciences, Malmö
  • Diabetes - Immunovirology
  • Genomics, Diabetes and Endocrinology
  • Diabetes and Celiac Unit

Publishing year

2007

Language

English

Pages

521-526

Publication/Series

Diabetic Medicine

Volume

24

Issue

5

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Endocrinology and Diabetes

Keywords

  • autoimmunity
  • therapy
  • Type 1 diabetes

Status

Published

Research group

  • Diabetes - Immunovirology
  • Genomics, Diabetes and Endocrinology
  • Diabetes and Celiac Unit

ISBN/ISSN/Other

  • ISSN: 1464-5491