Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here:

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

ludc web

Anna Wendt

Assistant researcher

ludc web

MiR-335 regulates exocytotic proteins and affects glucose-stimulated insulin secretion through decreased Ca2+-dependent exocytosis in beta cells


  • V.A. Salunkhe
  • J. Ofori
  • N.R. Gandasi
  • S.A. Salö
  • A. Wendt
  • S. Barg
  • J.L.S. Esguerra
  • L. Eliasson

Summary, in English

Background and aims: Ca2+-induced exocytosis is essential for insulin to be secreted from beta-cells, and in islets from type-2 diabetic (T2D) donors the expression of several genes coding for exocytotic proteins is reduced. Largely this phenomenon cannot be explained by polymorphism; rather it is likely due to epigenetic factors like microRNAs (miRNAs). Indeed, previous studies have identified a number of miRNAs with differential expression in the islets from T2D donors and the Goto- Kakizaki (GK) rat. One of the upregulatedmiRNAs in the GK rat is miR- 335, predicted to target several exocytotic genes amongst those Stxbp1 is a validated target. Here we aim to investigate whether miR-335 regulates the expression of exocytotic genes and affects insulin secretion and exocytosis in beta-cells. Materials and methods: Insulin secretion was measured by radio immuno assay. Exocytosis and docking of insulin granules was studied by capacitance measurements using the patch-clamp technique and by TIRF microscopy. Rat miR-335 was overexpressed using chemicallymodified mature microRNA mimic in INS-1 832/13 beta-cells by transfection. Gene knockdown was performed with RNAi. Protein and mRNA levels were analysed with Western Blot and RT-qPCR, respectively. Results: Overexpression of miR-335 (OE335) in INS-1 832/13 cells reduced insulin secretion at 16.7 mM glucose compared to control cells (SCR) (n=3; p


  • Diabetes - Islet Cell Exocytosis
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year











Suppl. 1

Document type

Conference paper: abstract




  • Endocrinology and Diabetes


  • glucose
  • protein
  • insulin
  • microRNA
  • synaptotagmin
  • nitrogen 15
  • messenger RNA
  • insulin release
  • exocytosis
  • European
  • diabetes mellitus
  • gene
  • microscopy
  • donor
  • membrane
  • Goto Kakizaki rat
  • rat
  • genetic transfection
  • patch clamp technique
  • immunoassay
  • gene silencing
  • pancreas islet beta cell
  • density
  • electric potential
  • Western blotting
  • adaptation
  • cell function
  • telecommunication



Research group

  • Diabetes - Islet Cell Exocytosis


  • ISSN: 1432-0428