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ludc web

Anna Wendt

Assistant researcher

ludc web

MiR-335 regulates exocytotic proteins and affects glucose-stimulated insulin secretion through decreased Ca2+-dependent exocytosis in beta cells

Author

  • V.A. Salunkhe
  • J. Ofori
  • N.R. Gandasi
  • S.A. Salö
  • A. Wendt
  • S. Barg
  • J.L.S. Esguerra
  • L. Eliasson

Summary, in English

Background and aims: Ca2+-induced exocytosis is essential for insulin to be secreted from beta-cells, and in islets from type-2 diabetic (T2D) donors the expression of several genes coding for exocytotic proteins is reduced. Largely this phenomenon cannot be explained by polymorphism; rather it is likely due to epigenetic factors like microRNAs (miRNAs). Indeed, previous studies have identified a number of miRNAs with differential expression in the islets from T2D donors and the Goto- Kakizaki (GK) rat. One of the upregulatedmiRNAs in the GK rat is miR- 335, predicted to target several exocytotic genes amongst those Stxbp1 is a validated target. Here we aim to investigate whether miR-335 regulates the expression of exocytotic genes and affects insulin secretion and exocytosis in beta-cells. Materials and methods: Insulin secretion was measured by radio immuno assay. Exocytosis and docking of insulin granules was studied by capacitance measurements using the patch-clamp technique and by TIRF microscopy. Rat miR-335 was overexpressed using chemicallymodified mature microRNA mimic in INS-1 832/13 beta-cells by transfection. Gene knockdown was performed with RNAi. Protein and mRNA levels were analysed with Western Blot and RT-qPCR, respectively. Results: Overexpression of miR-335 (OE335) in INS-1 832/13 cells reduced insulin secretion at 16.7 mM glucose compared to control cells (SCR) (n=3; p

Department/s

  • Diabetes - Islet Cell Exocytosis
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2015-09-01

Language

English

Pages

128-128

Publication/Series

Diabetologia

Volume

58

Issue

Suppl. 1

Document type

Conference paper: abstract

Publisher

Springer

Topic

  • Endocrinology and Diabetes

Keywords

  • glucose
  • protein
  • insulin
  • microRNA
  • synaptotagmin
  • nitrogen 15
  • messenger RNA
  • insulin release
  • exocytosis
  • European
  • diabetes mellitus
  • gene
  • microscopy
  • donor
  • membrane
  • Goto Kakizaki rat
  • rat
  • genetic transfection
  • patch clamp technique
  • immunoassay
  • gene silencing
  • pancreas islet beta cell
  • density
  • electric potential
  • Western blotting
  • adaptation
  • cell function
  • telecommunication

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis

ISBN/ISSN/Other

  • ISSN: 1432-0428