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ludc web

Anna Wendt

Assistant researcher

ludc web

Importance of the 3' untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme

Author

  • E Lövkvist Wallström
  • K Takao
  • A Wendt
  • C Vargiu
  • H Yin
  • Lo Persson

Summary, in English

Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated feedback control of ODC synthesis. The hypotonic induction of ODC, on the other hand, was shown to be highly dependent on the presence of the 3' UTR, but not on the 5' UTR, of ODC mRNA. Cells expressing ODC mRNAs lacking the 3' UTR showed no, or only a very slight, induction of ODC whether the 5' UTR was present or not, whereas the cell lines expressing ODC mRNAs containing the 3' UTR (with or without the 5' UTR) markedly induced ODC after a hypotonic shock. The present finding of a role for the ODC mRNA 3' UTR in the hypotonic induction of ODC is the first demonstration of a specific effect of the 3' UTR in the regulation of ODC.

Department/s

  • Department of Experimental Medical Science
  • Diabetes - Islet Cell Exocytosis
  • Biogenic Amines

Publishing year

2001-06

Language

English

Pages

34-627

Publication/Series

The Biochemical journal

Volume

356

Issue

2

Document type

Journal article

Publisher

Portland Press

Topic

  • Cell and Molecular Biology

Keywords

  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Animals
  • CHO Cells
  • Cricetinae
  • Down-Regulation/drug effects
  • Enzyme Induction
  • Hypotonic Solutions
  • Ornithine Decarboxylase/biosynthesis
  • Protein Biosynthesis
  • Putrescine/metabolism
  • RNA, Messenger/genetics
  • Spermidine/metabolism
  • Spermine/metabolism

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis
  • Biogenic Amines

ISBN/ISSN/Other

  • ISSN: 0264-6021