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Anja Schmidt-Christensen

Assistant researcher

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Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Author

  • Corinne Berclaz
  • Daniel Szlag
  • David Nguyen
  • Jérôme Extermann
  • Arno Bouwens
  • Paul J. Marchand
  • Julia Nilsson
  • Anja Schmidt-Christensen
  • Dan Holmberg
  • Anne Grapin-Botton
  • Theo Lasser

Summary, in English

In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.

Department/s

  • Autoimmunity

Publishing year

2016-11-01

Language

English

Pages

4569-4580

Publication/Series

Biomedical Optics Express

Volume

7

Issue

11

Document type

Journal article

Publisher

Optical Society of America

Topic

  • Radiology, Nuclear Medicine and Medical Imaging
  • Endocrinology and Diabetes

Keywords

  • Biology
  • Flow diagnostics
  • Optical coherence tomography
  • Three-dimensional image acquisition

Status

Published

Project

  • inflammatory events leading to autoimmune Diabetes
  • 3D imaging of inflammation and autoimmunity

Research group

  • Autoimmunity

ISBN/ISSN/Other

  • ISSN: 2156-7085