Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Anja Schmidt-Christensen

Assistant researcher

Default user image.

E2-2 Dependent Plasmacytoid Dendritic Cells Control Autoimmune Diabetes.

Author

  • Lisbeth Hansen
  • Anja Schmidt-Christensen
  • Shashank Gupta
  • Nina Fransén Pettersson
  • Tine Hannibal
  • Boris Reizis
  • Pere Santamaria
  • Dan Holmberg

Summary, in English

Autoimmune diabetes is a consequence of immune-cell infiltration and destruction of pancreatic β-cells in the islets of Langerhans. We analyzed the cellular composition of the insulitic lesions in the autoimmune-prone non-obese diabetic (NOD) mouse and observed a peak in recruitment of plasmacytoid dendritic cells (pDCs) to NOD islets around 8-9 weeks of age. This peak coincides with increased spontaneous expression of type-1-IFN response genes and CpG1585 induced production of IFN-α from NOD islets. The transcription factor E2-2 is specifically required for the maturation of pDCs, and we show that knocking out E2-2 conditionally in CD11c+ cells leads to a reduced recruitment of pDCs to pancreatic islets and reduced CpG1585 induced production of IFN-α during insulitis. As a consequence, insulitis has a less aggressive expression profile of the Th1 cytokine IFN-γ and a markedly reduced diabetes incidence. Collectively, these observations demonstrate a disease-promoting role of E2-2 dependent pDCs in the pancreas during autoimmune diabetes in the NOD mouse.

Department/s

  • Autoimmunity
  • Department of Experimental Medical Science
  • Genomics, Diabetes and Endocrinology
  • Stem Cell Center

Publishing year

2015-12-01

Language

English

Publication/Series

PLoS ONE

Volume

10

Issue

12

Document type

Journal article

Publisher

Public Library of Science

Topic

  • Immunology in the medical area

Status

Published

Project

  • inflammatory events leading to autoimmune Diabetes

Research group

  • Autoimmunity
  • Genomics, Diabetes and Endocrinology

ISBN/ISSN/Other

  • ISSN: 1932-6203