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Anja Schmidt-Christensen

Assistant researcher

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Optical projection tomography for rapid whole mouse brain imaging

Author

  • David Nguyen
  • Paul J. Marchand
  • Arielle L. Planchette
  • Julia Nilsson
  • Miguel Sison
  • Jérôme Extermann
  • Antonio Lopez
  • Marcin Sylwestrzak
  • Jessica Sordet-Dessimoz
  • Anja Schmidt-Christensen
  • Dan Holmberg
  • Dimitri Van De Ville
  • Theo Lasser

Summary, in English

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

Department/s

  • Autoimmunity

Publishing year

2017-12-01

Language

English

Pages

5637-5650

Publication/Series

Biomedical Optics Express

Volume

8

Issue

12

Document type

Journal article

Publisher

Optical Society of America

Topic

  • Radiology, Nuclear Medicine and Medical Imaging

Status

Published

Project

  • 3D imaging of inflammation and autoimmunity

Research group

  • Autoimmunity

ISBN/ISSN/Other

  • ISSN: 2156-7085