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Comparison of measurements of autoantibodies to glutamic acid decarboxylase and islet antigen-2 in whole blood eluates from dried blood spots using the RSR-enzyme linked immunosorbent assay kits and in-house radioimmunoassays.

Author:
  • Anders Persson
  • Charlotte Becker
  • Ida Jönsson
  • Anita Ramelius
  • Carina Törn
Publishing year: 2010
Language: English
Publication/Series: Experimental Diabetes Research
Volume: 2010
Document type: Journal article
Publisher: Hindawi Publishing Corporation

Abstract english

To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P < .001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.

Keywords

  • Endocrinology and Diabetes

Other

Published
  • Clinical Chemistry, Malmö
  • Diabetes and Celiac Unit
  • ISSN: 1687-5214
E-mail: anita [dot] ramelius [at] med [dot] lu [dot] se

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