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Two insulin gene single nucleotide polymorphisms associated with type 1 diabetes risk in the Finnish and Swedish populations

Author:
  • Antti-Pekka Laine
  • Hanna Holmberg
  • Anita Ramelius
  • E. Ortqvist
  • Minna Kiviniemi
  • Outi Vaarala
  • Hans K. Akerblom
  • Olli Simell
  • Mikael Knip
  • Johnny Ludvigsson
  • Sten Ivarsson
  • Karin Larsson
  • Åke Lernmark
  • Jorma Ilonen
Publishing year: 2007
Language: English
Pages: 139-145
Publication/Series: Disease Markers
Volume: 23
Issue: 3
Document type: Journal article
Publisher: IOS Press

Abstract english

We have developed high-throughput tests for the detection of the insulin gene region SNPs -23HphI and -2221MspI. The potential of these markers to enhance the efficiency of type I diabetes risk screening was then evaluated by analyzing them in Finnish and Swedish populations. Blood spots on filter paper were analyzed using PCR followed by sequence- specific hybridization and time-resolved fluorometry reading. Distribution of the genotypes at both positions differed significantly among the affected children compared to the controls. The risk genotypes (CC, AA) were significantly more common in Finland than in Sweden, both among patients and controls. The VNTR genotype homozygous for the protective class III alleles showed a significantly stronger protective effect than the heterozygote (p = 0.02). Analyzing both SNPs enabled the detection of VNTR class III subclasses IIIA and IIIB. The observed significance between effects of the protective genotypes was due to the strong protective effect of the IIIA/IIIA genotype. IIIA/IlIA was the only genotype with significant discrepancy between protective effects compared to the other class III genotypes. These observations suggest that heterogeneity between the protective IDDM2 lineages could exist, and analyzing both -23HphI and -2221MspI would thus potentially enhance the sensitivity and specificity of type I diabetes risk estimation.

Keywords

  • Endocrinology and Diabetes
  • screening for
  • Finnish population
  • type 1 diabetes
  • insulin gene region
  • genetic risk

Other

Published
  • Diabetes and Celiac Unit
  • ISSN: 0278-0240
E-mail: anita [dot] ramelius [at] med [dot] lu [dot] se

Biomedical analyst

Diabetes and Celiac Unit

+46 40 39 19 06

91:10:018

Jan Waldenströms gata 35, Malmö

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Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00