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Alexander Perfilyev

Assistant researcher

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Liver DNA methylation of FADS2 associates with FADS2 genotypex 06 Biological Sciences 0604 Genetics

Author

  • Paula Walle
  • Ville Männistö
  • Vanessa Derenji De Mello
  • Maija Vaittinen
  • Alexander Perfilyev
  • Kati Hanhineva
  • Charlotte Ling
  • Jussi Pihlajamäki

Summary, in English

Background: Non-alcoholic fatty liver disease has been associated with increased mRNA expression of FADS2 in the liver and estimated activity of delta-6 desaturase in serum, encoded by the FADS2 gene. Since DNA methylation in the FADS1/2/3 gene cluster has been previously linked with genetic variants and desaturase activities, we now aimed to discover factors regulating DNA methylation of the CpG sites annotated to FADS1/2 genes. Methods: DNA methylation levels in the CpG sites annotated to FADS2 and FADS1 were analyzed from liver samples of 95 obese participants of the Kuopio Obesity Surgery Study (34 men and 61 women, age 49.5 ± 7.7 years, BMI 43.0 ± 5.7 kg/m2) using the Infinium HumanMethylation450 BeadChip (Illumina). Associations between DNA methylation levels and estimated delta-6 and delta-5 desaturase enzyme activities, liver histology, hepatic mRNA expression, FADS1/2 genotypes, and erythrocyte folate levels were analyzed. Results: We found a negative correlation between DNA methylation levels of cg06781209 and cg07999042 and hepatic FADS2 mRNA expression (both p < 0.05), and with estimated delta-6 desaturase activity based on both liver and serum fatty acids (all p < 0.05). Interestingly, the methylation level of cg07999042 (p = 0.001) but not of cg06781209 (p = 0.874) was associated with FADS2 variant rs174616. Conclusions: Genetic variants of FADS2 may contribute to the pathogenesis of non-alcoholic fatty liver disease by modifying DNA methylation.

Department/s

  • Diabetes - Epigenetics
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2019-01-17

Language

English

Publication/Series

Clinical Epigenetics

Volume

11

Issue

1

Document type

Journal article

Publisher

BioMed Central (BMC)

Topic

  • Endocrinology and Diabetes
  • Medical Genetics

Keywords

  • Delta-6 desaturase
  • DNA methylation
  • Epigenetics
  • FADS2
  • Liver
  • mRNA expression
  • Non-alcoholic fatty liver disease

Status

Published

Research group

  • Diabetes - Epigenetics

ISBN/ISSN/Other

  • ISSN: 1868-7075