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Alexander Perfilyev

Assistant researcher

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Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Author

  • Christa Broholm
  • Anders Henrik Olsson
  • Alexander Perfilyev
  • Linn Gillberg
  • Ninna Schiøler Hansen
  • Ashfaq Ali
  • Brynjulf Mortensen
  • Charlotte Ling
  • Allan Vaag

Summary, in English

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

Department/s

  • Diabetes - Epigenetics
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2016-12-01

Language

English

Pages

1601-1617

Publication/Series

Epigenomics

Volume

8

Issue

12

Document type

Journal article

Publisher

Future Medicine Ltd.

Topic

  • Endocrinology and Diabetes
  • Medical Genetics

Keywords

  • adipogenesis
  • adiponectin
  • DNA methylation
  • epigenetics
  • stem cells

Status

Published

Research group

  • Diabetes - Epigenetics

ISBN/ISSN/Other

  • ISSN: 1750-1911