
Alexander Perfilyev
Assistant researcher

DNA methylation patterns in human adipose tissue in relation to diet and type 2 diabetes
Author
Summary, in English
In study 1 we investigated whether the DNA methylation pattern in human adipose tissue is affected differently by a 7-week exposure to a diet high in either polyunsaturated fatty acids or saturated fatty acids. We found differences in methylation related to general fat overfeeding as well as between the two dietary groups.
In study 2 we studied the effect of 5 days of overfeeding with a high fat diet on DNA methylation in adipose tissue compared to a control diet. The participants were either born with a normal or low birth weight and it allowed also to compare the effects of the diets on these two groups separately. There were differences in methylation between subjects born with a low compared with normal birth weight.
In study 3 we used adipose tissue of a cohort of 14 monozygotic twin pairs discordant for type 2 diabetes in order to eliminate most of known confounding factors in comparing DNA methylation patterns as well as a case- control cohort for T2D. We found numerous differences in methylation in adipose tissue from subjects with T2D compared with controls, while less differences were found in the discordant twins.
In study 4 we compared DNA methylation in adipose-derived stem and differentiated cells in subjects born with a low or normal birth weight. We found differences in gene expression in adipose-derived stem cells between low birth weight and normal birth weight groups but did not identify differences in methylation patterns possibly due to lack of statistical power.
Overall, these studies contribute to a better understanding of the influence of environmental factors and type 2 diabetes on DNA methylation in human adipose tissue.
Department/s
- Diabetes - Epigenetics
- EXODIAB: Excellence of Diabetes Research in Sweden
Publishing year
2018
Language
English
Publication/Series
Lund University, Faculty of Medicine Doctoral Dissertation Series
Volume
2018:136
Issue
136
Full text
Document type
Dissertation
Publisher
Lund University: Faculty of Medicine
Topic
- Endocrinology and Diabetes
Keywords
- DNA Methylation
- Adipose Tissue
- Illumina 450k methylation array
- type 2 diabetes
Status
Published
Research group
- Diabetes - Epigenetics
Supervisor
- Charlotte Ling
- Emma A Nilsson
- Tina Rönn
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-7619-704-2
Defence date
20 November 2018
Defence time
13:00
Defence place
Aulan, CRC, Jan Waldenströms gata 35, Skånes Universitetssjukhus i Malmö
Opponent
- Andrew P. Feinberg (professor)