Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Åke Lernmark

Principal investigator

Default user image.

In vivo effects of interleukin-1/β on blood leukocytes in bb rats prone or resistant to diabetes

Author

  • Lance W. Jobe
  • Sarah Vertrees
  • Cindy A. Wilson
  • Cindy Jacobs
  • Deborah L. Wilson
  • Kathleen S. Picha
  • Paul Bakert
  • Åke Lernmark

Summary, in English

Previous studies have determined that daily low dose injections of the potent cytokine interleukin-1β (IL-1β) decreased the frequency of insulin-dependent diabetes mellitus (IDDM) in diabetes-prone (DP) BB rats. In contrast, high dose injections induced an earlier than normal onset. In this study we tested whether the effects of daily human recombinant IL-1β injections on leukocyte subsets were associated with its modulation of IDDM onset in BB rats. Prior to the onset of IDDM in DP BB rats, high dose IL-1 β induced leukocytosis (P < 0.05), neutrophilia (P<0.01), and monocytosis (P < 0.001). At the onset of IDDM, lymphocyte (P < 0.01) and neutrophil (P<0.001) numbers were increased in high dose treated DP rats but not in rats given saline or low dose IL-1β. In 60-day-old diabetes-resistant (DR) BB rats, neurophilia was induced by both low (P<0.05) and high (P<0.001) dose IL-1 β without the development of IDDM. At 130 days of age, when the rats were killed, it was discovered that 14/22 (64% IL-1β injected DR rats developed neutralizing IL-1β antibodies. Significantly lower neutrophil numbers were observed in high dose DR rats which developed IL-1β antibodies compared with those which did not (P=0.032). Thus, neutrophilia was dissociated from high IL-1β acceleration of IDDM onset. These results suggest that acceleration of diabetogenesis in DP rats by IL-1β is associated with increased prediabetic levels of monocytes at 60 days of age and of lymphocytes at onset of IDDM.

Publishing year

1992-01-01

Language

English

Pages

233-237

Publication/Series

Autoimmunity

Volume

11

Issue

4

Document type

Journal article

Publisher

Taylor & Francis

Keywords

  • IL-1β antibodies
  • Insulin-dependent diabetes
  • Leukocytosis
  • Lymphocytosis
  • Monocytosis
  • Neutrophilia

Status

Published

ISBN/ISSN/Other

  • ISSN: 0891-6934