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Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay

Author:
  • Dong Luo
  • Chad N Rogers
  • Christiane S Hampe
  • Åke Lernmark
Publishing year: 2003
Language: English
Pages: 249-255
Publication/Series: Journal of Biotechnology
Volume: 103
Issue: 3
Document type: Journal article
Publisher: Elsevier

Abstract english

We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.

Keywords

  • Endocrinology and Diabetes

Other

Published
  • ISSN: 1873-4863
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

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