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Next-generation sequencing for viruses in children with rapid-onset type 1 diabetes

Author:
  • H-S Lee
  • T. Briese
  • C. Winkler
  • M. Rewers
  • E. Bonifacio
  • H. Hyoty
  • M. Pflueger
  • O. Simell
  • J. X. She
  • W. Hagopian
  • Åke Lernmark
  • B. Akolkar
  • J. P. Krischer
  • A. G. Ziegler
Publishing year: 2013
Language: English
Pages: 1705-1711
Publication/Series: Diabetologia
Volume: 56
Issue: 8
Document type: Journal article
Publisher: Springer

Abstract english

Viruses are candidate causative agents in the pathogenesis of autoimmune (type 1) diabetes. We hypothesised that children with a rapid onset of type 1 diabetes may have been exposed to such agents shortly before the initiation of islet autoimmunity, possibly at high dose, and thus study of these children could help identify viruses involved in the development of autoimmune diabetes. We used next-generation sequencing to search for viruses in plasma samples and examined the history of infection and fever in children enrolled in The Environmental Determinants of Diabetes in the Young (TEDDY) study who progressed to type 1 diabetes within 6 months from the appearance of islet autoimmunity, and in matched islet-autoantibody-negative controls. Viruses were not detected more frequently in plasma from rapid-onset patients than in controls during the period surrounding seroconversion. In addition, infection histories were found to be similar between children with rapid-onset diabetes and control children, although episodes of fever were reported less frequently in children with rapid-onset diabetes. These findings do not support the presence of viraemia around the time of seroconversion in young children with rapid-onset type 1 diabetes.

Keywords

  • Endocrinology and Diabetes
  • GB virus C
  • Human herpes virus 6
  • Human rhinovirus C
  • Infections
  • Islet
  • autoimmunity
  • Next-generation deep sequencing
  • Parvovirus B19
  • TEDDY
  • study
  • Type 1 diabetes mellitus

Other

Published
  • Diabetes and Celiac Unit
  • ISSN: 1432-0428
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

+46 40 39 19 01

+46 70 616 47 79

60:11:015

Jan Waldenströms gata 35, Malmö

33

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00