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Åke Lernmark

Principal investigator

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Relating homology between the Epstein-Barr virus BOLF1 molecule and HLA-DQw8 β chain to recent onset Type 1 (insulin-dependent) diabetes mellitus

Author

  • T. Sairenji
  • M. Daibata
  • C. H. Sorli
  • H. Qvistbäck
  • R. E. Humphreys
  • J. Ludvigsson
  • J. Palmer
  • M. Landin-Olsson
  • G. Sundkvist
  • B. Michelsen
  • Å Lernmark
  • T. Dyrberg

Summary, in English

A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed DQw8-positive lymphocytes in an immunoblotting analysis. Epstein-Barr virus antibodies were detected at equal frequencies and similar titres in sera of 30 patients with Type 1 diabetes (16 of 30;63%) and in sera of 20 non-diabetic control subjects (13 of 20;65%). Sera from diabetic patients did not bind to DQw8 β (44-63) or BOLF1(496-515) peptides. From these data we conclude that there is no simple relationship between serological evidence of Epstein-Barr virus infection and crossreactions between homologous Epstein-Barr virus and class II major histocompatibility complex peptides.

Department/s

  • Diabetes lab
  • Medicine, Lund
  • Department of Clinical Sciences, Malmö

Publishing year

1991-01-01

Language

English

Pages

33-39

Publication/Series

Diabetologia

Volume

34

Issue

1

Document type

Journal article

Publisher

Springer

Topic

  • Endocrinology and Diabetes

Keywords

  • class II MHC molecules
  • EBV BOLF1
  • Epstein-Barr virus
  • molecular mimicry
  • Type 1 (insulin-dependent) diabetes mellitus

Status

Published

Research group

  • Diabetes lab

ISBN/ISSN/Other

  • ISSN: 0012-186X