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Dynamic perifusion to maintain and assess isolated pancreatic islets.

Author:
  • I R Sweet
  • D L Cook
  • R W Wiseman
  • C J Greenbaum
  • Åke Lernmark
  • S Matsumoto
  • J C Teague
  • K A Krohn
Publishing year: 2002
Language: English
Pages: 67-76
Publication/Series: Diabetes Technology & Therapeutics
Volume: 4
Issue: 1
Document type: Journal article
Publisher: Mary Ann Liebert, Inc.

Abstract english

Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells were adequately supplied with oxygen and nutrients and remained functionally responsive. Isolated human and monkey islets that were perifused for 18 h showed robust biphasic insulin secretion in response to a step increase in glucose, demonstrating the a

Keywords

  • Endocrinology and Diabetes

Other

Published
  • ISSN: 1520-9156
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

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+46 70 616 47 79

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Jan Waldenströms gata 35, Malmö

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Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00