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Åke Lernmark

Principal investigator

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Flow sorting of mouse pancreatic B cells by forward and orthogonal light scattering

Author

  • O. Nielsen
  • J. K. Larsen
  • I. J. Christensen
  • Lernmark

Summary, in English

Mouse islet cell suspensions were separated into subpopulations based on forward low angle and orthogonal scattered light. Separated fractions were analyzed for hormone content by radioimmunoassays. The pancreatic B‐cells had the highest orthogonal light scattering, while they overlapped in forward low angle light scattering with other endocrine (glucagon, pancreatic polypeptide and somatostatin containing cells) and nonendocrine cells. Living and paraformaldehyde fixed islet cell suspensions showed similar distributions of combined light scattering.

Publishing year

1982-01-01

Language

English

Pages

177-181

Publication/Series

Cytometry

Volume

3

Issue

3

Document type

Journal article

Publisher

John Wiley and Sons

Keywords

  • flow cytometry
  • Insulin‐containing cells
  • mouse
  • orthogonal light scattering
  • subpopulation separation

Status

Published

ISBN/ISSN/Other

  • ISSN: 0196-4763