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Åke Lernmark

Principal investigator

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Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Author

  • Susanne Linde
  • Bruno Hansen
  • Åke Lernmark

Summary, in English

Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

Publishing year

1980-01-01

Language

English

Pages

165-176

Publication/Series

Analytical Biochemistry

Volume

107

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Endocrinology and Diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 0003-2697