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Åke Lernmark

Principal investigator

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The use of dispersed pancreatic islet cells in measurements of transmembrane transport

Author

  • Åke Lernmark
  • Janove Sehlin
  • Inge Bert Täljedal

Summary, in English

Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca2+-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb+ about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the study of transport kinetics in the pancreatic islet cells.

Publishing year

1975-01-01

Language

English

Pages

73-79

Publication/Series

Analytical Biochemistry

Volume

63

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Endocrinology and Diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 0003-2697