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Åke Lernmark

Principal investigator

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A simple and rapid microSepharose assay for GAD65 and ICA512 autoantibodies in diabetes

Author

  • Dorota B. Schranz
  • Lynn Bekris
  • Mona Landin-Olsson
  • Carina Törn
  • Anna Niläng
  • Åsa Toll
  • Hans Grönlund
  • Bert Toivola
  • Åke Lernmark

Summary, in English

GAD65Ab and ICA512Ab are strongly associated with insulin-dependent (Type 1) diabetes mellitus. A novel, simple radio-antigen binding assay with microSepharose conjugated with monoclonal antibodies specific for human immunoglobulin light chains was developed to provide diagnostic sensitivity and specificity of GAD65Ab and ICA512Ab for Type 1 diabetes. The Receiver Operating Characteristic (ROC) curve was used to determine the upper level of Normal in 583 new onset Type 1 diabetic patients and in 829 matched controls. The sensitivity of GAD65Ab and ICA512Ab was 66% (384/583) and 41% (211/520), respectively, and the diagnostic specificity was 96% for both autoantibodies. Levels, but not frequency, of GAD65Ab were higher among female Type 1 diabetes patients, whereas ICA512Ab levels did not differ between males and females. Positivity for GAD65Ab, ICA512Ab or both showed a sensitivity of 74% and a specificity of 92% for Type 1 diabetes. This simple, one-step centrifugation, high-capacity radio-antigen binding assay has a high precision and reproducibility to accurately detect both GAD65Ab and ICA512Ab. This assay should also prove useful in other autoantibody assays against conformation-sensitive autoantigens.

Publishing year

1998-04-01

Language

English

Pages

87-97

Publication/Series

Journal of Immunological Methods

Volume

213

Issue

1

Document type

Journal article

Publisher

Elsevier

Keywords

  • Autoimmunity
  • Islet cell autoantibodies
  • MicroSepharose
  • Radio-antigen binding assay
  • ROC curve
  • Type 1 diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-1759